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Lampire Biological chicken red blood cells lampire biological
Chicken Red Blood Cells Lampire Biological, supplied by Lampire Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chicken+red+blood+cells+lampire+biological/chicken+red+blood+cells+lampire+biological/pm40073270-58-0-4
Average 90 stars, based on 1 article reviews
chicken red blood cells lampire biological - by Bioz Stars, 2026-09
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Incubation:

Article Title: RIG-I and TLR-7/8 agonists as combination adjuvant shapes unique antibody and cellular vaccine responses to seasonal influenza vaccine.
Article Snippet: .. The sera-virus mixture was incubated with 0.5% chicken red blood cells (Lampire Biological Laboratories, USA) for 30 min at 4°C or until the blood coagulated. ..

Article Title: Stability kinetics of influenza vaccine coated onto microneedles during drying and storage.
Article Snippet: .. To determine HA activity titers, inactivated influenza virus in solution form or dissolved from metal chips was serially diluted in 100 μL of PBS deficient in Mg2+ and Ca2+, mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories, Pipersville, PA), and incubated for 1 h at 25°C. ..

Article Title: Enhanced Stability of Inactivated Influenza Vaccine Encapsulated in Dissolving Microneedle Patches
Article Snippet: .. Then, 25 μl aliquots of two-fold serially diluted serum samples were mixed with 25 μl virus solution containing four hemagglutinin units of A/Puerto Rico/8/34 influenza virus at 37°C and incubated for 1 h, followed by incubation with 50 μl of 0.5% chicken red blood cells (LAMPIRE Biological Laboratories) for 45 min at 25°C. ..

Article Title: Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
Article Snippet: Briefly, mouse sera were heat-inactivated at 56°C for 1 h and then treated with receptor destroying enzyme (Denka Seiken, Tokyo, Japan) at 37°C overnight according to the manufacturer’s instruction. .. After treatment, 25-μl aliquots of 2-fold serially diluted serum samples were added to 25-μl PBS containing 4 HA units of purified inactivated recombinant influenza virus, After incubation at 37°C for 1 h, the serum-virus mixture was then incubated with 50 μl of 0.5% chicken red blood cells (LAMPIRE Biological Laboratories, Pipersville, PA) at 25°C for 45 min in a u-bottom 96-well plate. ..

Article Title: Intranasal Immunization with Inactivated Influenza Virus Enhances Immune Responses to Coadministered Simian-Human Immunodeficiency Virus-Like Particle Antigens
Article Snippet: .. To determine HA titers, inactivated influenza virus was serially diluted in 100 l of PBS deficient in Mg2 and Ca2 , mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories), and incubated for 2 h at 4°C. ..

Article Title: Intranasal Immunization with Inactivated Influenza Virus Enhances Immune Responses to Coadministered Simian-Human Immunodeficiency Virus-Like Particle Antigens
Article Snippet: .. To determine HA titers, inactivated influenza virus was serially diluted in 100 μl of PBS deficient in Mg 2+ and Ca 2+ , mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories), and incubated for 2 h at 4°C. ..

Virus:

Article Title: Local Response to Microneedle-Based Influenza Immunization in the Skin
Article Snippet: .. To assess virus titers, hemagglutination (HA) activity was determined using chicken red blood cells (LAMPIRE Biological Laboratories, Pipersville, PA) and by a protein concentration assay (Bio-Rad Laboratories, Hercules, CA), as previously described ( ). .. Inactivated PR8 virus was labeled using the EZ-link sulfo NHS-L-C biotin reagent (Thermoscientific, Rockford, IL) following the manufacturer’s instructions, followed by labeling with streptavidin Qdot 655 or Qdot 705 (Invitrogen, Eugene, OR).

Article Title: Stability kinetics of influenza vaccine coated onto microneedles during drying and storage.
Article Snippet: .. To determine HA activity titers, inactivated influenza virus in solution form or dissolved from metal chips was serially diluted in 100 μL of PBS deficient in Mg2+ and Ca2+, mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories, Pipersville, PA), and incubated for 1 h at 25°C. ..

Article Title: Enhanced Stability of Inactivated Influenza Vaccine Encapsulated in Dissolving Microneedle Patches
Article Snippet: .. Then, 25 μl aliquots of two-fold serially diluted serum samples were mixed with 25 μl virus solution containing four hemagglutinin units of A/Puerto Rico/8/34 influenza virus at 37°C and incubated for 1 h, followed by incubation with 50 μl of 0.5% chicken red blood cells (LAMPIRE Biological Laboratories) for 45 min at 25°C. ..

Article Title: Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
Article Snippet: Briefly, mouse sera were heat-inactivated at 56°C for 1 h and then treated with receptor destroying enzyme (Denka Seiken, Tokyo, Japan) at 37°C overnight according to the manufacturer’s instruction. .. After treatment, 25-μl aliquots of 2-fold serially diluted serum samples were added to 25-μl PBS containing 4 HA units of purified inactivated recombinant influenza virus, After incubation at 37°C for 1 h, the serum-virus mixture was then incubated with 50 μl of 0.5% chicken red blood cells (LAMPIRE Biological Laboratories, Pipersville, PA) at 25°C for 45 min in a u-bottom 96-well plate. ..

Article Title: Intranasal Immunization with Inactivated Influenza Virus Enhances Immune Responses to Coadministered Simian-Human Immunodeficiency Virus-Like Particle Antigens
Article Snippet: .. To determine HA titers, inactivated influenza virus was serially diluted in 100 l of PBS deficient in Mg2 and Ca2 , mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories), and incubated for 2 h at 4°C. ..

Article Title: Intranasal Immunization with Inactivated Influenza Virus Enhances Immune Responses to Coadministered Simian-Human Immunodeficiency Virus-Like Particle Antigens
Article Snippet: .. To determine HA titers, inactivated influenza virus was serially diluted in 100 μl of PBS deficient in Mg 2+ and Ca 2+ , mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories), and incubated for 2 h at 4°C. ..

Activity Assay:

Article Title: Local Response to Microneedle-Based Influenza Immunization in the Skin
Article Snippet: .. To assess virus titers, hemagglutination (HA) activity was determined using chicken red blood cells (LAMPIRE Biological Laboratories, Pipersville, PA) and by a protein concentration assay (Bio-Rad Laboratories, Hercules, CA), as previously described ( ). .. Inactivated PR8 virus was labeled using the EZ-link sulfo NHS-L-C biotin reagent (Thermoscientific, Rockford, IL) following the manufacturer’s instructions, followed by labeling with streptavidin Qdot 655 or Qdot 705 (Invitrogen, Eugene, OR).

Article Title: Stability kinetics of influenza vaccine coated onto microneedles during drying and storage.
Article Snippet: .. To determine HA activity titers, inactivated influenza virus in solution form or dissolved from metal chips was serially diluted in 100 μL of PBS deficient in Mg2+ and Ca2+, mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories, Pipersville, PA), and incubated for 1 h at 25°C. ..

Protein Concentration:

Article Title: Local Response to Microneedle-Based Influenza Immunization in the Skin
Article Snippet: .. To assess virus titers, hemagglutination (HA) activity was determined using chicken red blood cells (LAMPIRE Biological Laboratories, Pipersville, PA) and by a protein concentration assay (Bio-Rad Laboratories, Hercules, CA), as previously described ( ). .. Inactivated PR8 virus was labeled using the EZ-link sulfo NHS-L-C biotin reagent (Thermoscientific, Rockford, IL) following the manufacturer’s instructions, followed by labeling with streptavidin Qdot 655 or Qdot 705 (Invitrogen, Eugene, OR).

Suspension:

Article Title: Stability kinetics of influenza vaccine coated onto microneedles during drying and storage.
Article Snippet: .. To determine HA activity titers, inactivated influenza virus in solution form or dissolved from metal chips was serially diluted in 100 μL of PBS deficient in Mg2+ and Ca2+, mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories, Pipersville, PA), and incubated for 1 h at 25°C. ..

Article Title: Intranasal Immunization with Inactivated Influenza Virus Enhances Immune Responses to Coadministered Simian-Human Immunodeficiency Virus-Like Particle Antigens
Article Snippet: .. To determine HA titers, inactivated influenza virus was serially diluted in 100 l of PBS deficient in Mg2 and Ca2 , mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories), and incubated for 2 h at 4°C. ..

Article Title: Intranasal Immunization with Inactivated Influenza Virus Enhances Immune Responses to Coadministered Simian-Human Immunodeficiency Virus-Like Particle Antigens
Article Snippet: .. To determine HA titers, inactivated influenza virus was serially diluted in 100 μl of PBS deficient in Mg 2+ and Ca 2+ , mixed with an equal volume of a fresh 0.5% suspension of chicken red blood cells (Lampire Biological Laboratories), and incubated for 2 h at 4°C. ..

Purification:

Article Title: Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
Article Snippet: Briefly, mouse sera were heat-inactivated at 56°C for 1 h and then treated with receptor destroying enzyme (Denka Seiken, Tokyo, Japan) at 37°C overnight according to the manufacturer’s instruction. .. After treatment, 25-μl aliquots of 2-fold serially diluted serum samples were added to 25-μl PBS containing 4 HA units of purified inactivated recombinant influenza virus, After incubation at 37°C for 1 h, the serum-virus mixture was then incubated with 50 μl of 0.5% chicken red blood cells (LAMPIRE Biological Laboratories, Pipersville, PA) at 25°C for 45 min in a u-bottom 96-well plate. ..

Recombinant:

Article Title: Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates
Article Snippet: Briefly, mouse sera were heat-inactivated at 56°C for 1 h and then treated with receptor destroying enzyme (Denka Seiken, Tokyo, Japan) at 37°C overnight according to the manufacturer’s instruction. .. After treatment, 25-μl aliquots of 2-fold serially diluted serum samples were added to 25-μl PBS containing 4 HA units of purified inactivated recombinant influenza virus, After incubation at 37°C for 1 h, the serum-virus mixture was then incubated with 50 μl of 0.5% chicken red blood cells (LAMPIRE Biological Laboratories, Pipersville, PA) at 25°C for 45 min in a u-bottom 96-well plate. ..



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R848 adjuvantation does not improve the neutralizing capacity or avidity of IAV-specific antibodies after prime/boost vaccination in elderly AGM. ( A ) The neutralizing capacity of IAV-specific antibodies was assessed by HAI assays at d0, d10 p.v. and d10 p.b. Serially diluted plasma was incubated <t>with</t> <t>PR8</t> for 30 min and then transferred to chicken red blood cells for hemagglutination assessment. HAI titer is defined by the highest dilution at which the sample prevents agglutination. Each animal is designated a distinct symbol; these symbols are consistent across time points to allow for longitudinal visualization of each individual response. IPR8- and IPR8-R848-vaccinated animals are designated by black or grey symbols, respectively. ( B ) The average avidity of PR8-specific IgG at d21 p.b. was calculated by determining the NaSCN concentration that gave a 50% reduction in optical absorbance compared to the untreated sample. Seven animals were included in each vaccine group, and the averages shown are the mean ±SEM. Statistical significance was determined using a two-way ANOVA with repeated measures and Tukey’s multiple comparisons test ( A ) or an unpaired two-tailed t-test ( B ). No statistical significance was observed, and the p -value was >0.05.
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R848 adjuvantation does not improve the neutralizing capacity or avidity of IAV-specific antibodies after prime/boost vaccination in elderly AGM. ( A ) The neutralizing capacity of IAV-specific antibodies was assessed by HAI assays at d0, d10 p.v. and d10 p.b. Serially diluted plasma was incubated <t>with</t> <t>PR8</t> for 30 min and then transferred to chicken red blood cells for hemagglutination assessment. HAI titer is defined by the highest dilution at which the sample prevents agglutination. Each animal is designated a distinct symbol; these symbols are consistent across time points to allow for longitudinal visualization of each individual response. IPR8- and IPR8-R848-vaccinated animals are designated by black or grey symbols, respectively. ( B ) The average avidity of PR8-specific IgG at d21 p.b. was calculated by determining the NaSCN concentration that gave a 50% reduction in optical absorbance compared to the untreated sample. Seven animals were included in each vaccine group, and the averages shown are the mean ±SEM. Statistical significance was determined using a two-way ANOVA with repeated measures and Tukey’s multiple comparisons test ( A ) or an unpaired two-tailed t-test ( B ). No statistical significance was observed, and the p -value was >0.05.
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R848 adjuvantation does not improve the neutralizing capacity or avidity of IAV-specific antibodies after prime/boost vaccination in elderly AGM. ( A ) The neutralizing capacity of IAV-specific antibodies was assessed by HAI assays at d0, d10 p.v. and d10 p.b. Serially diluted plasma was incubated <t>with</t> <t>PR8</t> for 30 min and then transferred to chicken red blood cells for hemagglutination assessment. HAI titer is defined by the highest dilution at which the sample prevents agglutination. Each animal is designated a distinct symbol; these symbols are consistent across time points to allow for longitudinal visualization of each individual response. IPR8- and IPR8-R848-vaccinated animals are designated by black or grey symbols, respectively. ( B ) The average avidity of PR8-specific IgG at d21 p.b. was calculated by determining the NaSCN concentration that gave a 50% reduction in optical absorbance compared to the untreated sample. Seven animals were included in each vaccine group, and the averages shown are the mean ±SEM. Statistical significance was determined using a two-way ANOVA with repeated measures and Tukey’s multiple comparisons test ( A ) or an unpaired two-tailed t-test ( B ). No statistical significance was observed, and the p -value was >0.05.
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R848 adjuvantation does not improve the neutralizing capacity or avidity of IAV-specific antibodies after prime/boost vaccination in elderly AGM. ( A ) The neutralizing capacity of IAV-specific antibodies was assessed by HAI assays at d0, d10 p.v. and d10 p.b. Serially diluted plasma was incubated <t>with</t> <t>PR8</t> for 30 min and then transferred to chicken red blood cells for hemagglutination assessment. HAI titer is defined by the highest dilution at which the sample prevents agglutination. Each animal is designated a distinct symbol; these symbols are consistent across time points to allow for longitudinal visualization of each individual response. IPR8- and IPR8-R848-vaccinated animals are designated by black or grey symbols, respectively. ( B ) The average avidity of PR8-specific IgG at d21 p.b. was calculated by determining the NaSCN concentration that gave a 50% reduction in optical absorbance compared to the untreated sample. Seven animals were included in each vaccine group, and the averages shown are the mean ±SEM. Statistical significance was determined using a two-way ANOVA with repeated measures and Tukey’s multiple comparisons test ( A ) or an unpaired two-tailed t-test ( B ). No statistical significance was observed, and the p -value was >0.05.
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R848 adjuvantation does not improve the neutralizing capacity or avidity of IAV-specific antibodies after prime/boost vaccination in elderly AGM. ( A ) The neutralizing capacity of IAV-specific antibodies was assessed by HAI assays at d0, d10 p.v. and d10 p.b. Serially diluted plasma was incubated <t>with</t> <t>PR8</t> for 30 min and then transferred to chicken red blood cells for hemagglutination assessment. HAI titer is defined by the highest dilution at which the sample prevents agglutination. Each animal is designated a distinct symbol; these symbols are consistent across time points to allow for longitudinal visualization of each individual response. IPR8- and IPR8-R848-vaccinated animals are designated by black or grey symbols, respectively. ( B ) The average avidity of PR8-specific IgG at d21 p.b. was calculated by determining the NaSCN concentration that gave a 50% reduction in optical absorbance compared to the untreated sample. Seven animals were included in each vaccine group, and the averages shown are the mean ±SEM. Statistical significance was determined using a two-way ANOVA with repeated measures and Tukey’s multiple comparisons test ( A ) or an unpaired two-tailed t-test ( B ). No statistical significance was observed, and the p -value was >0.05.
Chicken Red Blood Cells (Lampire Biological Labs), supplied by Lampire Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chicken+red+blood+cells+lampire+biological/chicken+red+blood+cells/pmc03375405-47-16-20
Average 90 stars, based on 1 article reviews
chicken red blood cells (lampire biological labs) - by Bioz Stars, 2026-09
90/100 stars
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R848 adjuvantation does not improve the neutralizing capacity or avidity of IAV-specific antibodies after prime/boost vaccination in elderly AGM. ( A ) The neutralizing capacity of IAV-specific antibodies was assessed by HAI assays at d0, d10 p.v. and d10 p.b. Serially diluted plasma was incubated with PR8 for 30 min and then transferred to chicken red blood cells for hemagglutination assessment. HAI titer is defined by the highest dilution at which the sample prevents agglutination. Each animal is designated a distinct symbol; these symbols are consistent across time points to allow for longitudinal visualization of each individual response. IPR8- and IPR8-R848-vaccinated animals are designated by black or grey symbols, respectively. ( B ) The average avidity of PR8-specific IgG at d21 p.b. was calculated by determining the NaSCN concentration that gave a 50% reduction in optical absorbance compared to the untreated sample. Seven animals were included in each vaccine group, and the averages shown are the mean ±SEM. Statistical significance was determined using a two-way ANOVA with repeated measures and Tukey’s multiple comparisons test ( A ) or an unpaired two-tailed t-test ( B ). No statistical significance was observed, and the p -value was >0.05.

Journal: Vaccines

Article Title: Analysis of R848 as an Adjuvant to Improve Inactivated Influenza Vaccine Immunogenicity in Elderly Nonhuman Primates

doi: 10.3390/vaccines10040494

Figure Lengend Snippet: R848 adjuvantation does not improve the neutralizing capacity or avidity of IAV-specific antibodies after prime/boost vaccination in elderly AGM. ( A ) The neutralizing capacity of IAV-specific antibodies was assessed by HAI assays at d0, d10 p.v. and d10 p.b. Serially diluted plasma was incubated with PR8 for 30 min and then transferred to chicken red blood cells for hemagglutination assessment. HAI titer is defined by the highest dilution at which the sample prevents agglutination. Each animal is designated a distinct symbol; these symbols are consistent across time points to allow for longitudinal visualization of each individual response. IPR8- and IPR8-R848-vaccinated animals are designated by black or grey symbols, respectively. ( B ) The average avidity of PR8-specific IgG at d21 p.b. was calculated by determining the NaSCN concentration that gave a 50% reduction in optical absorbance compared to the untreated sample. Seven animals were included in each vaccine group, and the averages shown are the mean ±SEM. Statistical significance was determined using a two-way ANOVA with repeated measures and Tukey’s multiple comparisons test ( A ) or an unpaired two-tailed t-test ( B ). No statistical significance was observed, and the p -value was >0.05.

Article Snippet: The RDE-treated plasma was then serially diluted and was incubated with 8 HAU/well of PR8 for 30 min before transferring to chicken red blood cells (cRBC) (Lampire Biologicals) for 45 min on ice.

Techniques: Clinical Proteomics, Incubation, Agglutination, Concentration Assay, Two Tailed Test